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P5380

Protease from Bacillus licheniformis

Type VIII, lyophilized powder, 7-15 units/mg solid

Synonym(s):

Proteinase from Bacillus licheniformis, Subtilisin A

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25 mg
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$90.10
100 mg
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$189.00
250 mg
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$285.00
1 g
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$860.00

About This Item

CAS Number:
9014-01-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-752-2
MDL number:
MFCD00132092
EC Number:
3.4.21.62 (BRENDA, IUBMB)
eCl@ss:
32160410
Specific activity:
7-15 units/mg solid
Biological source:
Bacillus licheniformis

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biological source

Bacillus licheniformis

Quality Level

200

type

Type VIII

form

lyophilized powder

specific activity

7-15 units/mg solid

mol wt

27 kDa

purified by

crystallization

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear, H2O: soluble, colorless

foreign activity

DNase ≤5.0 Kunitz units/mg solid, RNase ≤0.05 Kunitz units/mg solid

storage temp.

−20°C

General description

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
The enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60 °C.

Application

Non-specific Protease Activity Assay Video
The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination.[1] It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies.[2]
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.

Biochem/physiol Actions

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease, Product P5380, is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is active in some organic solvents such as dry octane.

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

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This Item
P4860P5459P8038
Protease from Bacillus licheniformis Type VIII, lyophilized powder, 7-15 units/mg solid

Sigma-Aldrich

P5380

Protease from Bacillus licheniformis

Protease from Bacillus licheniformis ≥2.4 U/g

Sigma-Aldrich

P4860

Protease from Bacillus licheniformis

Protease from Bacillus licheniformis glycerol solution (50%)

Sigma-Aldrich

P5459

Protease from Bacillus licheniformis

Proteinase, bacterial Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

Sigma-Aldrich

P8038

Proteinase, bacterial

biological source

Bacillus licheniformis

biological source

-

biological source

Bacillus licheniformis

biological source

-

specific activity

7-15 units/mg solid

specific activity

≥2.4 U/g

specific activity

≥7 units/mg protein (biuret)

specific activity

≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid

form

lyophilized powder

form

aqueous solution

form

glycerol solution (50%)

form

lyophilized powder

mol wt

27 kDa

mol wt

-

mol wt

-

mol wt

27 kDa

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear, H2O: soluble, colorless

solubility

-

solubility

0.85% sodium chloride: soluble, clear (Diluted), H2O: miscible

solubility

-

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000468500
0000468499
0000468498
0000468497
0000468497

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Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Tommi Kotila et al.
Nature communications, 9(1), 1892-1892 (2018-05-16)
Actin polymerization powers key cellular processes, including motility, morphogenesis, and endocytosis. The actin turnover cycle depends critically on "re-charging" of ADP-actin monomers with ATP, but whether this reaction requires dedicated proteins in cells, and the underlying mechanism, have remained elusive.
Carley R Benton et al.
The Journal of physiology, 586(6), 1755-1766 (2008-02-02)
Peroxisome proliferator-activated receptors (PPARs) alter the expression of genes involved in regulating lipid metabolism. Rosiglitazone, a PPARgamma agonist, induces tissue-specific effects on lipid metabolism; however, its mode of action in skeletal muscle remains unclear. Since fatty acid translocase (FAT/CD36) was
L Proctor et al.
Gynecologic oncology, 146(3), 596-602 (2017-06-26)
We sought to determine whether DNA ploidy correlates with the four molecular subgroups of endometrial carcinoma (EC) as determined using ProMisE (Proactive Molecular Risk Classifier for Endometrial Cancer). 90 cases of EC previously characterized by clinicopathological parameters, outcomes, and ProMisE
View All Related Papers

Global Trade Item Number

SKUGTIN
P5380-250MG04061834379171
P5380-25MG04061834379232
P5380-1G04061835506583
P5380-100MG04061835558445

Questions

  1. Hi  How I can mix my enzyme with water?

    1 answer

    1. This product is soluble in water at normal usage concentrations.

      Kindly review the technical data sheet for concentration suggestions available at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/194/864/p5380pis-ms.pdf

      Helpful?

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