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Poly-ʟ-Lysine Hydrobromide

Name: Poly-ʟ-Lysine Hydrobromide
Brand: SIGMA
Price: 128 CAD

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Poly-L-lysine hydrobromide

Synonym(s):

PLL HBr

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Pack Size	SKU	Availability	Price
5 mg		Check Cart for Availability	$128.00

About This Item

CAS Number:

25988-63-0

UNSPSC Code:

12352202

eCl@ss:

32160406

PubChem Substance ID:

24898718

NACRES:

NA.75

MDL number:

MFCD00237230

Biological source:

synthetic (organic)

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

Mol wt:

≥300,000

$128.00

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Properties

Product Name

Poly-L-lysine hydrobromide, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

biological source

synthetic (organic)

Quality Level

200

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

≥300,000

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm²

impurities

<10% water (by Karl Fischer)

solubility

H₂O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

Description

Application

Poly-L-lysine hydrobromide has been used to coat:

2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation[1]
flat bottom 96-well plates for in vitro neuronal stem cell differentiation[2]
T75 flasks for primary astrocytic cultures[3]

Biochem/physiol Actions

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Disclaimer

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

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1 of 1

This Item	P1274	P4707	P8920
Sigma-Aldrich P5899 Poly-L-lysine hydrobromide	Sigma-Aldrich P1274 Poly-L-lysine hydrobromide	Sigma-Aldrich P4707 Poly-L-lysine solution	Sigma-Aldrich P8920 Poly-L-lysine solution
biological source synthetic (organic)	biological source -	biological source synthetic	biological source synthetic
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -
sterility γ-irradiated	sterility -	sterility sterile-filtered	sterility -
Quality Level 200	Quality Level 300	Quality Level 300	Quality Level 200
mol wt ≥300,000	mol wt 70,000-150,000 by viscosity	mol wt 70—150 kDa	mol wt 150,000-300,000
form lyophilized powder	form solid	form liquid	form -

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

Protocole d'adhésion cellulaire à la poly-L-Lysine

Utilisation de poly-lysine pour faire adhérer les cellules à des substrats solides, augmentant ainsi les interactions électrostatiques entre les ions chargés négativement de la membrane cellulaire et de la surface de culture.

Articles

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Global Trade Item Number

SKU	GTIN
P5899-5MG	04061834392569