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P5899

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Poly-L-lysine hydrobromide

Synonyme(s) :

PDL HBr

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A propos de cet article

Numéro CAS:
25988-63-0
UNSPSC Code:
12352202
eCl@ss:
32160406
PubChem Substance ID:
24898718
NACRES:
NA.75
MDL number:
MFCD00237230
Biological source:
synthetic (organic)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Mol wt:
≥300,000

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Nom du produit

Poly-L-lysine hydrobromide, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

biological source

synthetic (organic)

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

≥300,000

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

impurities

<10% water (by Karl Fischer)

solubility

H2O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

−20°C

Quality Level

200

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Cet article
P9155P1524P0879
Poly-L-lysine hydrobromide mol wt &#8805;300,000, lyophilized powder, &#947;-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

P5899

Poly-L-lysine hydrobromide

Poly-L-lysine hydrobromide mol wt 30,000-70,000, lyophilized powder, &#947;-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

P9155

Poly-L-lysine hydrobromide

Poly-L-lysine hydrobromide mol wt &#8805;300,000

Sigma-Aldrich

P1524

Poly-L-lysine hydrobromide

Poly-L-lysine hydrobromide mol wt 1,000-5,000

Sigma-Aldrich

P0879

Poly-L-lysine hydrobromide

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

γ-irradiated

sterility

γ-irradiated

sterility

-

sterility

-

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

-

biological source

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mol wt

≥300,000

mol wt

30,000-70,000

mol wt

≥300,000

mol wt

1,000-5,000

form

lyophilized powder

form

lyophilized powder

form

powder

form

solid

Application

Poly-L-lysine hydrobromide has been used to coat:
  • 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation[1]
  • flat bottom 96-well plates for in vitro neuronal stem cell differentiation[2]
  • T75 flasks for primary astrocytic cultures[3]

Biochem/physiol Actions

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces.[4] Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate.[5] Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.[5]

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

La poly-L-lysine est un polymère d′acide aminé à charge positive, comportant environ un HBr par résidu de lysine. Le bromhydrate permet de former une poudre cristalline de poly-L-lysine soluble dans l′eau. Une petite quantité de produit peut se trouver dans des structures bêta, car le HBr interfère avec la formation de liaisons d′hydrogène entre les groupes aminés et les groupes carboxyliques ou les parties contenant des atomes N ou O.
For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Disclaimer

Les solutions stériles sont stables pendant environ 2 ans si conservées entre 2 et 8 °C. Conserver à une température de -20  C en présence d′un déshydratant.

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000315801
SLCL5836
0000315801
SLCL5836
SLCF0030

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo
June Seok Heo, et al.
BioMed Research International (2016)
Gustavo Castro et al.
ISRN ophthalmology, 2013, 752161-752161 (2014-02-22)
Purpose. The purpose of this work was to evaluate the retinal integration and differentiation of neurospheres formed by stem cells and mouse neural progenitor cells injected intravitreally in mice eyes with retinal injury. Methods. Eight male C57BL mice, 8 weeks
Sonja Stadler et al.
BMC cell biology, 5(1), 44-44 (2004-11-24)
Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown. We compared the large-scale chromatin structure of
Polylysine for skin regeneration: A review of recent advances and future perspectives
Payam Zarrintaj, et al.
Journal of Separation Science, 5(7) (2021)
Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating
Alexander Faussner, et al.
PLoS ONE, 17(7) (2022)
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Poly-lysine Types for Biomedical Applications

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

Protocoles

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

Protocole d'adhésion cellulaire à la poly-L-Lysine

Utilisation de poly-lysine pour faire adhérer les cellules à des substrats solides, augmentant ainsi les interactions électrostatiques entre les ions chargés négativement de la membrane cellulaire et de la surface de culture.

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