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P6887

Pepsin from porcine gastric mucosa

lyophilized powder, ≥3,200 units/mg protein

Synonym(s):

Pepsin A, Pepsin from hog stomach

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250 mg

Available to ship TODAYfromSchnelldorf Distribution

682,00 kr.
1 g

Available to ship TODAYfromSchnelldorf Distribution

1.950,00 kr.
5 g

Available to ship TODAYfromSchnelldorf Distribution

5.550,00 kr.
10 g

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9.520,00 kr.

About This Item

CAS Number:
9001-75-6
UNSPSC Code:
12352204
eCl@ss:
42010127
EC Number:
232-629-3
NACRES:
NA.54
MDL number:
MFCD00081840
EC Number:
3.4.23.1 (BRENDA, IUBMB)
Specific activity:
≥3,200 units/mg protein
Biological source:
Porcine gastric mucosa

682,00 kr.

Available to ship TODAYDetails

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biological source

Porcine gastric mucosa

Quality Segment

300

grade

Proteomics Grade

form

lyophilized powder

specific activity

≥3,200 units/mg protein

mol wt

35 kDa

impurities

salt, essentially free

color

white to off-white

solubility

deionized water: soluble 10 mg/mL, 10 mM HCl: soluble 4.0 mg/mL (Cold)

UniProt accession no.

P00791

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Gene Information

pig ... LOC396892(396892)

Application

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.
Pepsin from Sigma has been used along with other enzymes for the determination of enzyme-resistant starch (RS) in bread. It has also been used to simulate in vitro gastrointestinal digestion of pea or whey protein isolates.
Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Product P6887 is provided as a lyophilized powder and has been used to digest protein during dietary fiber analysis.

Biochem/physiol Actions

Unlike many other peptidases, pepsin hydrolyzes only peptide bonds, not amide or ester linkages. The cleavage specificity includes peptides with an aromatic acid on either side of the peptide bond, especially if the other residue is also an aromatic or a dicarboxylic amino acid. Increased susceptibility to hydrolysis occurs if there is a sulfur-containing amino acid close to the peptide bond, which has an aromatic amino acid. Pepsin will also preferentially cleave at the carboxyl side of phenylalanine and leucine, and to a lesser extent at the carboxyl side of glutamic acid residues. It does not cleave at valine, alanine, or glycine linkages. Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion. Pepsin is inhibited by several phenylalanine-containing peptides.
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
Protein determined by E1%/280

Other Notes

One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37°C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 ml. Light path = 1 cm.)
View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.

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Pepsin from porcine gastric mucosa lyophilized powder, ≥3,200 units/mg protein

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P7000

Pepsin from porcine gastric mucosa

description

lyophilized powder, ≥3,200 units/mg protein

description

lyophilized powder, ≥2,500 units/mg protein (E1%/280)

description

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥3200 units/mg protein

description

powder, ≥250 units/mg solid

biological source

Porcine gastric mucosa

biological source

-

biological source

-

biological source

Porcine gastric mucosa

Gene Information

pig ... LOC396892(396892)

Gene Information

pig ... LOC396892(396892)

Gene Information

pig ... LOC396892(396892)

Gene Information

pig ... LOC396892(396892)

grade

Proteomics Grade

grade

-

grade

-

grade

-

specific activity

≥3,200 units/mg protein

specific activity

≥2,500 units/mg protein (E1%/280)

specific activity

≥3200 units/mg protein

specific activity

≥250 units/mg solid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

9001-75-6

CAS No.

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