The ReadyMix contains the following components: 20 mM Tris-HCl, pH 8.3, with 100 mM KCl, 3 mM MgCl2, 0.002% gelatin, 0.4 mM deoxynucleotide triphosphate mix (deoxyadenosine triphosphate, deoxycytidine triphosphate, deoxyguanosine triphosphate, thymidine triphosphate), stabilizers, and 0.06 unit/mL of Taq DNA Polymerase.
R2523
REDTaq® ReadyMix™ PCR Reaction Mix
Complete PCR reagent with standard Taq DNA Polymerase and inert dye
Synonym(s):
PCR master mix, PCR master mix with loading dye, Taq PCR master mix
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| Size/SKU | Availability | Price |
|---|---|---|
20 reactions | Available to ship TODAYfromLyon | €60.70 |
100 reactions | Available to ship TODAYfromLyon | €219.00 |
About This Item
NACRES:
NA.55
UNSPSC Code:
41106300
Quality Segment
form
liquid
usage
sufficient for 100 reactions, sufficient for 20 reactions
feature
dNTPs included, hotstart: no, Standard PCR
technique(s)
PCR: suitable using : suitable, WGA
color
red
input
purified DNA
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
General description
REDTaq® ReadyMix™ is a ready-to-use mixture of Taq DNA polymerase, 99% pure DNTPs, reaction buffer, and an inert red dye in a 2X concentrate. After the PCR reaction, the PCR product can be loaded directly onto an agarose gel. The red loading dye migrates at approximately the same rate as a 125 base pair fragment in a 1% agarose gel. The inert dye has no effect on the amplification process, and therefore, a sample can be easily re-amplified such as in “nested PCR”.
Application
Features and Benefits
The benefits of REDTaq® with the convenience of ReadyMix™
- Saves preparation time
- Reduces risk of contamination from multiple pipet steps and and increased reproducibility.
- Provides consistent reaction-to-reaction performance
- No loading buffer/tracking dye needs to be added. The PCR product is loaded directly into an agarose gel after amplification
- The red tracer migrates at approximately the same rate as a 125bp fragment (slightly faster than bromophenol blue)
- Amplifies targets up to 7 kb in length
- Because no loading buffer has been added, a sample can be easily re-amplifed such as in nested PCR.
- Stable at 4°C or −20°C.
- Ready-mix format reduces setup time.
Packaging
Default reaction volume is 50 μL
20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
Preparation Note
Add 25 μl of ReadyMix to template, primers and water to a final reaction volume of 50 μl. The PCR product is easily separated from the dye by standard purification methodologies. The inert red dye has no effect on automated sequencing and is suitable for restriction enzyme digestion, ligation and other downstream manipulations.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| description Complete PCR reagent with standard Taq DNA Polymerase and inert dye | description for PCR | description for High-throughput PCR of complex templates | description Ready-to-use 2X PCR Master Mix with Loading Dye |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| feature dNTPs included, Standard PCR, hotstart: no | feature dNTPs included, hotstart | feature Difficult Templates/Specialty Enzymes PCR, dNTPs included, hotstart | feature dNTPs included, hotstart |
| input purified DNA | input purified DNA | input purified DNA | input - |
| usage sufficient for 100 reactions, sufficient for 20 reactions | usage sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 800 reactions | usage sufficient for 100 reactions, sufficient for 400 reactions | usage sufficient for 100 reactions, 1.2 mL sufficient for 100 amplifications, sufficient for 1000 reactions, 12 mL sufficient for 1000 amplifications, sufficient for 10000 reactions, 125 mL sufficient for 10000 amplifications |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Storage Class
10 - Combustible liquids
wgk
WGK 1
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What's included and not included in the ReadyMix R2523
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Hello! For this ReadyMix (R2523), do I need to add MgCl2?
1 answer-
Yes, MgCl2 should be added to the ReadyMix. The ReadyMix contains the following components: 20 mM Tris-HCl, pH 8.3, with 100 mM KCl, 3 mM MgCl2, 0.002% gelatin, 0.4 mM deoxynucleotide triphosphate mix (deoxyadenosine triphosphate, deoxycytidine triphosphate, deoxyguanosine triphosphate, thymidine triphosphate), stabilizers, and 0.06 unit/mL of Taq DNA Polymerase.
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