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Anti-β-Tubulin III Antibody

Name: Anti-β-Tubulin III Antibody
Brand: SIGMA
Price: 556 USD
Availability: InStock

Enhanced Validation

rabbit polyclonal

Synonym(s):

Anti-Tuj 1

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$556.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01323870

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

448

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Properties

Product Name

Anti-β-Tubulin III antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

rat, human, mouse

enhanced validation

knockout
Learn more about Antibody Enhanced Validation

technique(s)

immunoprecipitation (IP): 10 μg using RIPA extract (250 μg) of cultured rat PC12 cells, indirect immunofluorescence: 10-20 μg/mL using rat PC12 cells, western blot: 0.2-0.4 μg/mL using whole extracts of mouse brain or cultured human neuroblastoma SH-SY5Y cells

UniProt accession no.

Q13509

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)

Description

General description

Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.
α/β-Tubulin, an integral component of microtubules, is present in almost all eukaryotic cells.
α/β-Tubulin occurs mostly as soluble (approx. 100-110 kDa) heterodimeric sets of α- and β-tubulin isotypes or as polymers in assembled microtubules. β-Tubulin III (also designated β-4 chain) is found in the brain and dorsal root ganglia and appears to be localized to neurons of the central and peripheral nervous system. β-tubulin III is also found in Sertoli cells of the testis, spermatozoa tails, certain lung cells, and apparently some breast stromal cells.

Immunogen

synthetic peptide corresponding to amino acid residues 441-450 of human β-tubulin III (Ala⁴⁴⁶ to Ser⁴⁴⁶ substitution) with N-terminal added cysteine, conjugated to KLH. The sequence is conserved in mammals.

Application

Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:

Immunofluorescence
Immunostaining
Western blotting

Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:

immunofluorescence
immunostaining
western blotting
indirect immunoperoxidase assay (IPA)

Biochem/physiol Actions

Anti-β-Tubulin III recognizes human, mouse,and rat β-tubulin III.

Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Storage and Stability: For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working concentrations by titration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

1 of 1

This Item	T8578	T3952	T8660
Sigma-Aldrich T2200 Anti-β-Tubulin III antibody produced in rabbit	Sigma-Aldrich T8578 Anti-β-Tubulin III (neuronal) antibody, Mouse monoclonal	Sigma-Aldrich T3952 Anti-β-Tubulin III antibody produced in rabbit	Sigma-Aldrich T8660 Monoclonal Anti-β-Tubulin III antibody produced in mouse
description affinity isolated antibody, buffered aqueous solution	description ~1.0 mg/mL, clone 2G10, purified from hybridoma cell culture	description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description clone SDL.3D10, ascites fluid
biological source rabbit	biological source mouse	biological source rabbit	biological source mouse
antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form ascites fluid
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt antigen ~55 kDa	mol wt antigen ~50 kDa	mol wt antigen ~50 kDa	mol wt -

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Storage Class

12 - Non Combustible Liquids

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Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions

3 months ago

Can I check the methods of antigen retrieval (solution, temperature, pressure, etc.) AND the recommend concentration of this ab for human tissue IF staining?

1 answer
1. Technical Support
  
  6 days ago
  
  This antibody is has not been tested for suitability in Immunihistochemistry;, a specific antigen retrieval protocol has not been developed. However, the following general protocol has been utilized for other antibodies to ?- and ?-tubulin classes II and III:
  1. Deparaffinize in xylene and rehydrate
  2. Apply heat-induced antigen retrieval for 15 min in 10 mmol/L citrate buffer (pH 7).
  3. Allow to cool at room temperature for 20 min
  4. Rinse tissue sections in PBS
  5. Treat with 5% H2O2 for 15 min to block endogenous peroxidase
  6. Wash with PBS
  7. Incubate with the primary antibody for 60 min at room temperature.
  8. Washing with PBS
  9. Incubate with secondary antibody at room temperature for 30 min
  Note that this is only a general protocol, optimization may be required. In addition, the following publication also provides information on Antigen Retrieval for use with this antibody:
  https://pmc.ncbi.nlm.nih.gov/articles/PMC4480425/#i0006-3363-85-5-934-b20
  This antibody has not been tested for suitability in Immunofluorescence in human tissues. As provided in the product datasheet, for indirect immunofluorescence, a working antibody concentration of 10-20 µg/ml is recommended using rat pheochromocytoma PC12 cells. Please see the following link to review additional information available in the product datasheet:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/457/t2200dat.pdf
  
  Helpful?
a year ago

what is the concentration of this product?

1 answer
1. Technical Support
  
  a year ago
  
  The concentration of this antibody varies from lot to lot and will have a range of 0.60 - 1.0 mg/mL. This information is reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
  https://www.sigmaaldrich.com/product/sigma/t2200#product-documentation
  
  Helpful?

Reviews

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Average Customer Ratings

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Ap11
Ap11

Heidelberg, Germany
Review 1

Votes 0
5 out of 5 stars.

Ap11

2 years ago

good antibody for immunostaing
Consistent results on mouse and human tissue (nerves of the pancreas) as well as primary culture of sensory neurons. usual usage on tissue and cells: 1:500 (in blocking solution: 7 to 10% Horse serum in PBS)

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