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A8580

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

Synonym(s):

Anti-Glutathione-S-Transferase, Anti-GST

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
agarose conjugate
Clone:
polyclonal
Application:
ELISA, IP
Citations:
6

biological source

rabbit

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

PBS suspension

mol wt

antigen 27.5 kDa

should not react with

porcine, bovine, human, rabbit, rat

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... GSTP1(2950)

General description

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.
The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Application

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.
Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Biochem/physiol Actions

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
SAB5700816G7781SAB4200692
Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit IgG fraction of antiserum, PBS suspension

Sigma-Aldrich

A8580

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

Anti-GSTP1 antibody produced in rabbit

Sigma-Aldrich

SAB5700816

Anti-GSTP1 antibody produced in rabbit

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

G7781

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal clone GST-2, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200692

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

conjugate

agarose conjugate

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

GST-2, monoclonal

form

PBS suspension

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable

technique(s)

immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

dot blot: suitable, indirect ELISA: 1:10,000, western blot: 1:2,000 using lysates of induced Escherichia coli expressing GST

technique(s)

ELISA: suitable, dot blot: suitable, immunoblotting: 0.5 μg/mL, immunofluorescence: suitable, immunoprecipitation (IP): suitable

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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