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Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

Name: Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, PBS suspension

Synonym(s):

Anti-Glutathione-S-Transferase, Anti-GST

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

agarose conjugate

Clone:

polyclonal

Application:

ELISA, IP

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

Properties

biological source

rabbit

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

PBS suspension

mol wt

antigen 27.5 kDa

should not react with

porcine, bovine, human, rabbit, rat

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... GSTP1(2950)

Description

General description

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Application

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.

Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Biochem/physiol Actions

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	ABS1653	HPA006311	WH0002952M1
$Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit IgG fraction of antiserum, PBS suspension$ Sigma-Aldrich A8580 Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit	Sigma-Aldrich ABS1653 Anti-GSTT1 Antibody	Sigma-Aldrich HPA006311 Anti-GSTK1 antibody produced in rabbit	Sigma-Aldrich WH0002952M1 Monoclonal Anti-GSTT1 antibody produced in mouse
biological source rabbit	biological source rabbit	biological source rabbit	biological source mouse
conjugate agarose conjugate	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form IgG fraction of antiserum	antibody form serum	antibody form affinity isolated antibody	antibody form purified immunoglobulin
shipped in wet ice	shipped in dry ice	shipped in wet ice	shipped in dry ice
clone polyclonal	clone polyclonal	clone polyclonal	clone 2E10-1B2, monoclonal
form PBS suspension	form -	form buffered aqueous glycerol solution	form buffered aqueous solution

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
A8580-1ML	04061837856242

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