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CA200

cAMP Enzyme Immunoassay Kit, Direct

Sinónimos:

cAMP detection kit

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NACRES:
NA.84
UNSPSC Code:
12161503
Technique(s):
ELISA: suitable
Species reactivity:
-
Application:
ELISA
Citations:
44


technique(s)

ELISA: suitable

Quality Segment

shipped in

wet ice

storage temp.

−20°C

General description

Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it.
Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase.

Application

Please refer to the attached Protocolfor details.
cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium[1] virulent and attenuated macrophages.[2] It has also been used to quantify cAMP synthesis in olfactory epithelium.[3]

Biochem/physiol Actions

Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states.

Other Notes

Allow reagents to warm at room temperature for 30 minutes prior to opening.

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Este artículo
CA201CG201CG200
shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Solo componentes del kit

Referencia del producto
Descripción

  • 0.1 M Hydrochloric Acid 1 x 30

  • Acetic Anhydride 1 x 1

  • cAMP-Alkaline Phosphatase Conjugate 1 x 5

  • cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic AMP Standard 1 x 0.5

  • Direct cAMP Assay Layout Sheet 1 x 1

Ver todo (14)

signalword

Danger

target_organs

Respiratory system

Clase de almacenamiento

3 - Flammable liquids

flash_point_f

120.2 °F

flash_point_c

49 °C

wgk

WGK 3

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3



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