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CASP3F

Caspase 3 Assay Kit, Fluorimetric

Synonym(s):

Caspase-3 Activity Fluorometric Kit

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1 kit
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$1,040.00

About This Item

UNSPSC Code:
12161503
EC Number:
247-454-8
NACRES:
NA.84

$1,040.00

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Quality Level

200

usage

sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Other Notes

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

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This Item
CASP3CAPT131APT165
Caspase 3 Assay Kit, Fluorimetric

Sigma-Aldrich

CASP3F

Caspase 3 Assay Kit, Fluorimetric

Caspase 3 Assay Kit, Colorimetric

Sigma-Aldrich

CASP3C

Caspase 3 Assay Kit, Colorimetric

Caspase 3 Colorimetric Activity Assay Kit, DEVD Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.

Sigma-Aldrich

APT131

Caspase 3 Colorimetric Activity Assay Kit, DEVD

Caspase 3 Colorimetric Activity Assay Kit, DEVD Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.

Sigma-Aldrich

APT165

Caspase 3 Colorimetric Activity Assay Kit, DEVD

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Gene Information

human ... CASP3(836)

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

100

usage

sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml)

usage

sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml)

usage

-

usage

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Kit Components Only

Product No.
Description
  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • A1086
    Ac-DEVD-AMC substrate 2.5 mg
    SDS
  • A0835
    Ac-DEVD-CHO inhibitor .5 mg
    SDS
  • A9891
    7-Amino-4-methylcoumarin standard 1 mg
    SDS

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000412229
0000412229
0000212536
0000212536
0000111687

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Global Trade Item Number

SKUGTIN
CASP3F-1KT04061833531594

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