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E0632

Enterokinase from porcine intestine

≥0.5 units/mg solid

Synonym(s):

Enteropeptidase

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About This Item

CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-761-1
NACRES:
NA.54
MDL number:
EC Number:
Specific activity:
≥0.5 units/mg solid
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form

salt-free, lyophilized powder

Quality Segment

specific activity

≥0.5 units/mg solid

mol wt

150 kDa

storage temp.

−20°C

Application

The enzyme from Sigma has been used for the activation of trypsinogen in order to measure the activity of trypsin in hog pancreas. The study showed that antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure of weanling pigs.
Enterokinase from porcine intestine has been used in a study to report a new experimental model of the anomalous pancreatico-biliary junction. Enterokinase from porcine intestine has also been used in a study to investigate the insulinotropic region of the gastric inhibitory polypeptide.

Biochem/physiol Actions

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

Other Notes

One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C.

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E0885A8376C3142
description

≥0.5 units/mg solid

description

lyophilized powder, ≥100 units/mg protein

description

Grade II, salt-free, lyophilized powder, 300-1,500 units/mg protein

description

(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

specific activity

≥0.5 units/mg solid

specific activity

≥100 units/mg protein

specific activity

300-1,500 units/mg protein

specific activity

≥40 units/mg protein

form

salt-free, lyophilized powder

form

lyophilized powder

form

salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

mol wt

150 kDa

mol wt

150 kDa

mol wt

-

mol wt

25 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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