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MTOX1012

CAR Knockout HepaRG™ Cells

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

Synonym(s):

CAR knockout human hepatocyte cells, HepaRG CAR KO

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Size/SKUAvailabilityPrice
1 vial
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375 000 HUF

About This Item

UNSPSC Code:
12352207
NACRES:
NA.81
Biological source:
human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

375 000 HUF

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Product Name

CAR Knockout HepaRG™ Cells, 1 vial

biological source

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

Quality Segment

200

form

liquid

storage temp.

−196°C

Gene Information

human ... ABCB1(1346307)

General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG CAR KO cells express the major xenobiotic sensors (PXR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Application

See technical bulletin for detailed protocols

Features and Benefits

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

  • Sigma′s HepaRG CAR Knockout (KO) allows conclusive identification of CYP2B6 induction through CAR activation.

Zinc finger nucleases (ZFN) mediated Knock-out of NR1I3 (CAR) gene.

  • The frame-shift mutation of NR1I3 gene was confirmed by fragment length analysis and DNA sequencing.
  • Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.

Analysis Note

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Legal Information

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Sigma-Aldrich

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description

1 vial

description

1 vial

description

one vial, from human female liver(hepatocarcinoma and hepatitis C tumor)

description

one vial

biological source

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

biological source

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

biological source

human female liver (hepatocarcinoma and hepatitis C tumor)

biological source

human female liver (hepatocarcinoma and hepatitis C tumor)

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Gene Information

human ... ABCB1(1346307)

Gene Information

human ... NR1I2(1337040)

Gene Information

human ... AHR(196)

Gene Information

human ... ABCC2(1244)

form

liquid

form

liquid

form

liquid

form

liquid

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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