MTOXRCSUP
RPTEC Complete Supplement
sterile-filtered, suitable for cell culture, add to 500 ML of basal medium
Synonym(s):
Basal media supplement for huRPTEC
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About This Item
NACRES:
NA.81
UNSPSC Code:
12352207
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
storage temp.
−20°C
General description
Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 30 mL of RPTEC complete media supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC). The final RPTEC complete media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).
Application
RPTEC Complete Supplement has been used in SA7K cell line culture for cell characterization.
Preparation Note
Prepare Complete Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-Glutamine (G7513), 30 mL of RPTEC Complete Supplement (MTOXRCSUP), 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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