The kit RAB0201 is specific for amino acids 108-128 (P01275). This includes the active forms 7-36a and 7-37, as well as the inactive cleaved form 9-36a.
RAB0201
GLP-1 EIA Kit
for serum, plasma, culture supernatant and cell lysates
Synonym(s):
Glucagon-like-peptide 1
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Please contact Customer Service for Availability | $748.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
$748.00
Please contact Customer Service for Availability
species reactivity
human, rat, mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, enzyme immunoassay: suitable
input
sample type cell lysate
sample type plasma
sample type serum
sample type culture supernatant(s)
assay range
inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.17 pg/mL
standard curve range: 0.1–1000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... GCG(2641)
General description
The GLP-1 Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting GLP-1 peptide based on the principle of Competitive Enzyme Immunoassay.
Immunogen
Glucagon-like Peptide 1 (GLP-1), synthetic peptide
Application
For research use only. Not for use in diagnostic procedures.
Biochem/physiol Actions
Glucagon-Like Peptide I (GLP1) controls the metabolism of glucose by inducing the secretion of insulin and suppressing that of glucagon. Studies in Indonesian population show that reduced levels of this protein are linked with increased susceptibility to type 2 diabetes mellitus.[1] Plasma levels of this hormone also show significant association with systolic and diastolic blood pressure levels.[2]
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
10 - Combustible liquids
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Ai-Jun Li et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 310(8), R724-R732 (2016-01-23)
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Jennifer O Adeghate et al.
Cells, 10(8) (2021-08-28)
The effects of early (5-day) onset of diabetes mellitus (DM) on retina ultrastructure and cellular bioenergetics were examined. The retinas of streptozotocin-induced diabetic rats were compared to those of non-diabetic rats using light and transmission electron microscopy. Tissue localization of
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Beumer J, et al.
Nature Cell Biology, 20(8), 909-909 (2018)
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Which forms of GLP-1 are measured by the GLP ELISA (RAB0201)? Is it specific to the active form 7-36aa?
1 answer-
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Hi, 1 unit GLP-1 EIA Kit can be used for how many sample of blood?
1 answer-
The GLP-1 EIA Kit includes a 96-well strip plate. The assay requires the addition of a blank, six standard curve points and a positive control. It is recommend to run all standards, controls and samples in duplicate. After the addition of the blank, standards and controls, each assay can run 44 samples in duplicate.
It is recommended to perform a 2-fold sample dilution for serum/plasma samples. The sample volume required per well is 100 ul of 1:2 diluted samples. Therefore 62.5 ul of starting sample volume is sufficient to run each sample in duplicate.
For more information on the product , please view the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/159/844/rab0201bul.pdf
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