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Our comprehensive offering of protein biology-related applications include support for western blotting, ELISA, protein labeling, protein detection, and protein quantification. Explore our powerful protein and immunoassay applications protocols, and resources to drive your research forward.    [![The diagram shows a Primary Antibody Conjugate with two connected shapes—one pink and one teal. These represent different parts of the conjugate.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/ELISA-Application-Direct.jpg "ELISA")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/elisa) [ELISA](https://www.sigmaaldrich.com/US/en/applications/protein-biology/elisa) [![The diagram shows a Enzyme Activity Assays with a flow diagram](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/enzyme-activity-290x180.jpg "Enzyme Activity Assays")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/enzyme-activity-assays) [Enzyme Activity Assays](https://www.sigmaaldrich.com/US/en/applications/protein-biology/enzyme-activity-assays) [![Yellow antibody with green antigens binding to it, set against a purple background with colorful lines representing different interactions or reactions.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/ae-flow-cytometry-290x180px.jpg "Flow Cytometry")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/flow-cytometry) [Flow Cytometry](https://www.sigmaaldrich.com/US/en/applications/protein-biology/flow-cytometry) [![A scientist is conducting an experiment using a blue electrophoresis chamber connected with red and black cables on a white laboratory bench.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/workflow-gel-electrophoresis.jpg "Gel Electrophoresis")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/gel-electrophoresis) [Gel Electrophoresis](https://www.sigmaaldrich.com/US/en/applications/protein-biology/gel-electrophoresis) [![The image is a comparative diagram illustrating two biological staining techniques: Indirect Immunohistochemistry and Immunofluorescence.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/indirect-immunohistochemistry.jpg "Immunohistochemistry")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/immunohistochemistry) [Immunohistochemistry](https://www.sigmaaldrich.com/US/en/applications/protein-biology/immunohistochemistry) [![A diagram representing protein pathway inhibition as an example of interrogation of protein pathways which involves chemical screening and treatment, looking at protein activity/enzyme activity, and protein pathway modulation.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/52550_Protein_Image_Purple_290x180_Final.jpg "Interrogation of Protein Pathways: Inhibition")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/interrogation-protein-pathways) [Interrogation of Protein Pathways](https://www.sigmaaldrich.com/US/en/applications/protein-biology/interrogation-protein-pathways) [![A spiral molecular representation of blue and yellow protein structures on a light background.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/33380-protein-lysis-and-extraction.jpg "Lysis & Protein Extraction")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/lysis-and-protein-extraction) [Lysis & Protein Extraction](https://www.sigmaaldrich.com/US/en/applications/protein-biology/lysis-and-protein-extraction) [![The image illustrates the process of immunoprecipitation, a technique used in molecular biology to selectively isolate a specific protein or nucleic acid from a complex mixture](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/chip-procedure-workflow.png "Protein & Nucleic Acid Interactions")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-and-nucleic-acid-interactions) [Protein & Nucleic Acid Interactions](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-and-nucleic-acid-interactions) [![Protein Concentration & Buffer Exchange](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/protein-concentration-2-290x180px.jpg "The image appears to be a simplified illustration of a water purification process.")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-concentration-and-buffer-exchange) [Protein Concentration & Buffer Exchange](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-concentration-and-buffer-exchange) [![Image has a vibrant yellow background with a large red circle. Inside the circle, there is intricate yellow calligraphy or artwork.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/protein-expression-290x180.jpg "Protein Expression")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-expression) [Protein Expression](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-expression) [![Image describes a two-step process for labeling and purifying proteins, Step 1: Labeling and Step 2: Purification.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/atto-dyes-figure-2.jpg "Protein Labeling & Modification")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-labeling-and-modification) [Protein Labeling & Modification](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-labeling-and-modification) [![It is a colorful representation of a complex molecular structure. It features multiple intertwined shapes, each with distinct colors like green, blue, red, and yellow.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/protein-mass-spectrometry.jpg "Protein Mass Spectrometry")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-mass-spectrometry) [Protein Mass Spectrometry](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-mass-spectrometry) [![Protein Pull-Down](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/protein-quantitation-290x180.jpg "Protein Pull-Down")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-pulldown) [Protein Pull-Down](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-pulldown) [![This diagram illustrates the process of extracting DNA from a solution using pipettes.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/protein-affinity-chromatography-290x180.jpg "Protein Purification")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-purification) [Protein Purification](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-purification) [![This image has a flow diagram illustrating a chemical reaction involving a protein and other compounds.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/protein-quantitation-steps.jpg "Protein Quantitation")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-quantitation) [Protein Quantitation](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-quantitation) [![This image appears to be a 3D model of a complex protein structure. The protein consists of multiple intertwined and folded strands, each colored differently to distinguish the various parts.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/protein-structure.jpg "Protein Structural Analysis")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-structural-analysis) [Protein Structural Analysis](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-structural-analysis) [![This image depicts a scientist performing an experiment.](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/applications/protein-biology/western-blotting-lab.jpg "Western Blotting")](https://www.sigmaaldrich.com/US/en/applications/protein-biology/western-blotting) [Western Blotting](https://www.sigmaaldrich.com/US/en/applications/protein-biology/western-blotting) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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