Life Science Reagents for Immunohistochemistry (IHC)

Find all the products you need to carry out successful Immunohistochemistry (IHC) in this one convenient location. All products are quality and availability assured. This bundle of Immunohistochemistry products includes:
  • Tissue Processing
  • Organic Solvents
  • Buffers
  • Blocking Sera and Reagents
  • Substrates for Alkaline Phosphatase
  • Substrates for Peroxidase
  • Avidin-Biotin Complex Reagents
  • Antibody Labeling
  • Antibody and Immunoassay Stabilizers
  • Antigen Retrieval Solutions
  • Routine Histology Stains
  • Additional Reagents
  • Lab Accessories

Prestige Antibodies® powered by Atlas Antibodies have 500+ publicly available immunohistological images for each antibody. For more information on Prestige Antibodies and IHC,
click here.

Additional Reagents

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A3648 (3-Aminopropyl)triethoxysilane ≥98%
F4680 Fluoromount Aqueous Mounting Medium for use with fluorescent dye-stained tissues
F6182 Fluoroshield histology mounting medium
F6057 Fluoroshield with DAPI histology mounting medium
F6937 Fluoroshield with 1,4-Diazabicyclo[2.2.2]octane
F5932 Fluoroshield with PI histology mounting medium
F6812 Fluoroshield with PPD
GG1 Glycerol Gelatin aqueous slide mounting medium
I1161 ImmunoHistoMount
O8015 Organo/Limonene Mount Green Alternative
P8920 Poly-L-lysine solution 0.1 % (w/v) in H2O
P5147 Protease from Streptomyces griseus Type XIV, ≥3.5 units/mg solid, powder
T7168 Trypsin from porcine pancreas tablet, 1 mg tablet

Antibody and Immunoassay Stabilizers

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A4955 Alkaline Phosphatase Stabilizing Buffer liquid
76696 BioStab Alkaline Phosphatase Conjugate Stabilizer
55514 BioStab Antibody Stabilizer
93859 BioStab immunoassay stabilizer

Antibody Labeling

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BTAG BiotinTag Micro Biotinylation Kit
FITC1 FluoroTag FITC Conjugation Kit
BK101 ImmunoProbe Biotinylation Kit

Antigen Retrieval Solutions

Antigen retrieval solutions improve the response of Immunohistothchemistry response by increasing the accessability of antibodies to tissue antigens. Processing formalin-fixed paraffin-embedded (FFPE) tissue sections with Antigen Retrieval solutions prior to application of antibodies can enhance immunostaining for low-expression proteins. Staining protocols can be optimized to reduce antibody concentration or incubation time.

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C9999 Citrate Buffer, pH 6.0, 10×, Antigen Retriever
E1161 EDTA Buffer, pH 8.5, 10×, Antigen Retriever
H3292 Histo/Zyme, pH 7.2, Ready to Use, Antigen Retriever
R2283 Pepsin Reagent, Ready to Use, Antigen Retriever
T6455 Tris-HCl Buffer, pH 10, 10×, Antigen Retriever

Avidin-Biotin Complex Reagents

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E2636 ExtrAvidin® –Alkaline Phosphatase buffered aqueous solution
E2886 ExtrAvidin®−Peroxidase buffered aqueous solution
EXTRA3 ExtrAvidin® Peroxidase Staining Kit for rabbit antibodies antibody produced in mouse
EXTRA2 Mouse ExtrAvidin® Peroxidase Staining Kit antibody produced in goat

Blocking Sera and Reagents

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A9647 Bovine Serum Albumin heat shock fraction, pH 7, ≥98%
B4501 Biotin ≥99% (TLC), lyophilized powder
G9023 Goat serum
H0146 Horse serum liquid
216763 Hydrogen peroxide solution contains inhibitor, 30 wt. % in H2O, ACS reagent Green Alternative
H1009 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer
M5905 Mouse serum
R9133 Rabbit serum
R9759 Rat serum
S3772 Sheep serum
L9756 (−)-Tetramisole hydrochloride ≥99% (GC)

Buffers

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18995 BioStab Peroxidase Conjugate Stabilizer
ED4SS Ethylenediaminetetraacetic acid tetrasodium salt dihydrate 99.0-102.0% (titration)
P9209 Peroxidase Stabilizing Buffer liquid
P3813 Phosphate buffered saline powder, pH 7.4, for preparing 1 L solutions
P4417 Phosphate buffered saline tablet
T5030 Tris Buffered Saline tablet
T6664 Tris Buffered Saline pH 8.0, powder
91414 Tris buffered saline with Tween® 20 BioUltra, tablet (for 500 mL), pH 7.6
T9039 Tris Buffered Saline, with Tween® 20, pH 8.0 powder
T1503 Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline
P1379 TWEEN® 20 viscous liquid

Lab Accessories

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C9802 Cover glasses size 22 mm × 22 mm
C7931 Cover glasses size 24 mm × 40 mm
C8181 Cover glasses size 24 mm × 50 mm
C9056 Cover glasses size 24 mm × 60 mm
Z265985 Dissecting scissors L 5 1/2 in., Mayo, straight
F4267 Dressing tissue forceps L 6 in.
Z252972 Enviro-Safe® thermometer -20-150 °C temperature, immersion level, 76 mm Green Alternative
H6644 Humidity chamber overall size 9 in. × 4 in., trays size 8 in. × 2 1/2 in. , with lid
Z380253 Jars Nalgene® style 2118 capacity 500 mL
Z188956 Kimwipes® disposable wipers L × W 4 1/2 in. × 8 1/2 in.
Z377821 PAP pen for immunostaining 5 mm tip width
Z672548 PAP pen for immunostaining 2 mm tip width
P0425 Poly-Prep Slides poly-L-lysine coated glass slides
S5641 Scienceware® Coplin staining jar with screw cap high-density polyethylene
Z648205 Securline lab marker II lab marker, for use on plastic embedding cassettes and frosted end of microscope slides, black, 5/cs
SL2 Sigmacote® siliconizing reagent for glass and other surfaces
S4651 Silane-Prep Slides glass slides coated with silane (aminoalkylsilane).
S8400 Slides, microscope frosted one end, size 25 mm × 75 mm
S9027 Slides, microscope opaque (white), size 25 mm × 75 mm
S8902 Slides, microscope plain, size 25 mm × 75 mm
Z648191 STATMARK permanent marker for cassette & slide marking pkg of 12 ea
Z672122 Tissue processing/embedding cassettes with lid pkg of 500 ea
Z719137 Wash Bottle capacity 500 mL (16 oz)
S5516 Wheaton Coplin staining jars 5 slide jar with ground glass cover
S5766 Wheaton Coplin staining jars 5 slide jar with plastic screw cap

Organic Solvents

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534064 Acetone histological grade, ≥99.5%
M1775 Methanol Absolute - Acetone free
R8382 Reagent Alcohol used for histology tissue preparation
R3404 Reagent Alcohol 95%, Used for histology tissue preparation
R3279 Reagent Alcohol 80%, used for histology tissue preparation
R3154 Reagent Alcohol 70%, used for histology tissue preparation
247642 Xylenes ACS reagent, ≥98.5% xylenes + ethylbenzene basis
534056 Xylenes histological grade
A5597 Xylene Substitute Green Alternative

Routine Histology Stains

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A3429 Differentiation Solution
A3179 Differentiation Solution
GHS116 Hematoxylin Solution, Gill No. 1
GHS132 Hematoxylin Solution, Gill No. 1
GHS1128 Hematoxylin Solution, Gill No. 1
GHS216 Hematoxylin Solution, Gill No. 2
GHS232 Hematoxylin Solution, Gill No. 2
GHS280 Hematoxylin Solution, Gill No. 2
GHS2128 Hematoxylin Solution, Gill No. 2
MHS16 Hematoxylin Solution, Mayer’s
MHS32 Hematoxylin Solution, Mayer’s
MHS80 Hematoxylin Solution, Mayer’s
MHS128 Hematoxylin Solution, Mayer’s
MHS1 Hematoxylin Solution, Mayer’s

Substrates for Alkaline Phosphatase

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B1911 BCIP®/NBT Liquid Substrate System ready to use solution
B5655 SIGMAFAST BCIP®/NBT tablet
F4648 SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets tablet, To prepare 1 mL
F4523 SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets tablet, To prepare 10 mL

Substrates for Peroxidase

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AEC101 AEC Staining Kit liquid
D3939 3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride for Immunohistology
D7304 3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride
D0426 SIGMAFAST DAB with Metal Enhancer tablet
D4168 SIGMAFAST 3,3′-Diaminobenzidine tablets tablet, To prepare 1 mL
D4293 SIGMAFAST 3,3′-Diaminobenzidine tablets tablet, To prepare 5 mL
D4418 SIGMAFAST 3,3′-Diaminobenzidine tablets tablet, To prepare 15 mL

Tissue Processing

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HT101128 Bouin’s solution
HT10132 Bouin’s solution
D0818 Decalcifying Solution-Lite aqueous solution, bp 208 °F
D3565 Declere solution 20× concentrate
45348 Epoxy embedding medium, accelerator ≥95% (NT)
45359 Epoxy Embedding Medium kit
A5472 Formalin Free Tissue Fixative Green Alternative
F5554 Formalin, 10%, Neutral Buffered, Wintergreen Scented
F5304 Formalin, 10%, Neutral Buffered with 0.03% Eosin
HT501128 Formalin solution, neutral buffered, 10% histological tissue fixative
HT501320 Formalin solution, neutral buffered, 10% histological tissue fixative
HT501640 Formalin solution, neutral buffered, 10% histological tissue fixative
G5882 Glutaraldehyde solution Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
H2904 HistoChoice® Tissue Fixative Green Alternative
158127 Paraformaldehyde powder, 95%
P6148 Paraformaldehyde reagent grade, crystalline
P3558 Paraplast® for tissue embedding
P3683 Paraplast Plus® for tissue embedding
P3808 Paraplast X-TRA® for tissue embedding
P6744 Picric acid solution 1.3% in H2O (saturated)
S4521 Saponin Quillaja sp. Sapogenin content 20-35 %
S1878 Sodium (meta)periodate ≥99.0%
A6330 Tissue Embedding Medium (Paraplast® Regular)
X100 Triton X-100 laboratory grade
Z2902 Zinc Formalin Fixative