CHOZN® Platform

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Accelerate Your Upstream Process Development Timelines

You need stable and off-the-shelf cell lines and media for the upstream development of your biologic and/or therapeutic proteins.

Powered by CompoZr® Zinc Finger Nuclease (ZFN) technology, our CHOZN® platform is the first ever commercially available Chinese Hamster Ovary (CHO) mammalian cell expression system that allows for faster, simpler selection and scale up of high producing clones for the production of recombinant protein drugs.

Our rationally-engineered expression platform includes a CHO cell line, paired media and feeds, as well as optimized processes that rapidly results in reliable, stable high titers in manufacturing clones, saving you up to 8 weeks in cell development timelines. Comprehensive user protocols guide you from transfection through small scale bioreactors, allowing for seamless scale up. What’s more, our CHOZN® platform is supported by full traceability and expert bioprocessing consultation and services, so you can accelerate your upstream process development timelines with ease.

CHOZN® Platform Key Features

  • Targeted gene modification allows for more powerful and faster clone selection when isolating producing cell lines
  • Optimized set of cGMP produced chemically defined growth and production media and feed developed to maximize growth and production of r-proteins from producing clones
  • Media and feed have been optimized to support initial cell line engineering as well as stable cell line selection through large scale growth and production
  • Media and feed formulations developed using animal component free raw materials, with a proven track record for sourcing and robust manufacturability
  • Stable platform with >90% of clones maintaining >75% titer of 60 generations

CHOZN® Platform Components

  • cGMP produced and tested CHOZN® GS-/- cell line (CHOGS)
  • Optimized vector for expression of antibodies, FC-fusion proteins or recombinant proteins
  • Detailed technical protocols from transfection to small scale bioreactors
  • Technical support from our CHOZN® Platform R&D team
  • One year research license
Product No. Description Product Information Sheet
CHOGS CHOZN® ZFN-modified GS-/- CHO Cell Line PIS (PDF)

Purchase the CHOZN® Platform through product number CHOGS. Please see CHOZN® Platform technical bulletin for more details.

The CHOZN® Omics Explorer

The CHOZN® GS-/-, fully-annotated, genome and transcriptome is available via a user-friendly, web-based interface, the CHOZN® Omics Explorer, that provides an unprecedented ability to characterize and genetically examine the host cell line and associated manufacturing clones. The full genetic and trancriptomic transparency will maximize your efficiency to:

  • Characterize transgene integration events with increased precision and confidence
  • Characterize and compare the host cell line with associated manufacturing clones
  • Better understand the underlying biology of favorable phenotypes
  • Plan complex genome engineering strategies
  • Design and utilize genome-wide screening tools
    Using our proprietary CHOZN® GS-/- cell line, a de novo genome, transcriptome assembly and annotation was performed utilizing second generation (Illumina®) sequencing, third generation long-read (PacBio® Sequel) sequencing as well as ultra-long range interaction mapping via sequencing of Hi-C and CHiCAGO® libraries.
   Access the CHOZN® Omics Explorer Here

Platform Media and Feeds

Optimized media and feeds that can be purchased separately as part of the CHOZN platform are listed below. Please see CHOZN Platform technical bulletin for details on media use.

Product No. Description Product Information Sheet
14366C EX-CELL® Advanced™ CHO Fed-batch Medium – Liquid PIS (PDF)
24366C EX-CELL Advanced™ CHO Fed-batch Medium – Powder PIS (PDF)
24367C EX-CELL Advanced CHO Feed 1 (with glucose) PIS (PDF)
24368C EX-CELL Advanced CHO Feed 1 (without glucose) PIS (PDF)
14365C EX-CELL CD CHO Fusion Medium
C6366 EX-CELL CHO Cloning Medium Poster (PDF)

Why Use CHOZN®?

Chinese Hamster Ovary (CHO) cell lines continue to be the predominant cell line of choice for biopharmaceutical manufacturers. SAFC’s CHOZN® products and services were created utilizing CompoZr™ Zinc Finger Nuclease (ZFN) technology to engineer CHO cell lines with characteristics that are attractive to biopharmaceutical developers and manufacturers, including stability, time, ease of use and adaptability.

Reliable – SAFC® has a long history providing our customers with the highest grade critical raw materials. We are your trusted supplier due to our quality systems & worldwide manufacturing capabilities. CHOZN® Products & Services continue SAFC’s history of excellence.

Decrease Development Timeline – Save up to 8 weeks in cell line development time lines (no MSX, no amplification, high rate of clone recovery, fewer clones screened)

Ease of Use – Comprehensive user protocols guide customers from transfection through small scale bioreactors; complete CD process allows for seamless scale up

Technical Support – CHOZN® GS Platform users gain access to the scientist who developed the cell lines. Prior to beginning cell line development, our team will meet your scientists to discuss protocols & share detailed information that ensures success. We maintain regular contact with our customers and are always available to discuss your project goals.

Stability – Stable platform with >90% of clones maintaining >75% titer of 60 generations

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Learn more about the CHOZN® Expression Platform
Download Technical Bulletin
Research product features
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