[Skip to Content](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[Protein & Nucleic Acid Interactions](https://www.sigmaaldrich.com/US/en/applications/protein-biology/protein-and-nucleic-acid-interactions)Using Duolink® in Protein Interaction Studies # Using Duolink® in Protein Interaction Studies The video above follows the simple and straightforward procedure that allows you to __detect, quantify and obtain cell localization__ of protein interactions and their modifications in a single experiment. - Cells or tissue deposited on slides or in microplates are fixed to preserve activation status and transient interactions. - Validated primary antibodies for the targets are added followed by binding of the PLA probes. - Hybridization of the oligonucleotide arms of the PLA probes will create a template for rolling circle amplification (RCA) only when the epitopes of the target proteins are in close proximity (<40 nm) - Amplification and labeling of the RCA product by detection probes. - The resulting spot can be detected and visualized by standard microscopy. - Images can easily be quantitatively analyzed using the Duolink Image Tool software, which facilitates both average and single cell data analysis. __Related Protocols__ - [RNA Immunoprecipitation qPCR (RIP- qPCR) Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/qpcr/rip-qpcr-protocol) - [Northern & Southern Blot Protocols](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/gel-electrophoresis/southern-and-northern-blotting) - [Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/anti-ago-rip-from-cells) - [Chromatin Isolation by RNA Purification (ChIRP) Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/chromatin-isolation-by-rna-purification) - [Creating PLA® Probes](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/creating-pla-probes-duolink-application-note) - [Duolink® PLA Brightfield Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/duolink-brightfield-user-manual) - [Counterstaining after the Duolink *In Situ* Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/duolink-counterstain) - [Duolink® PLA Flow Cytometry Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/duolink-cytometry-protocol) Top __Sign In To Continue__ To continue reading please sign in or create an account. Sign In__Don't Have An Account?__Register An unknown error has occured.