Analysis of Pesticides in Black Tea by GC/MS/MS on SLB(R) Using Supelclean(TM) Ultra Cleanup

Analysis of Pesticides in Black Tea by GC/MS/MS on SLB® Using Supelclean™ Ultra Cleanup


sample preparation sample pretreatment: 1 gm sample in 50 mL centrifuge tube, add 10 mL water
sample preparation Add QC check solution to each extract prior to GC/MS/MS analysis.
extraction tube Supel Que non-buffered extraction tube (55294-U)
clean-up process Add approx. 100 mg of annhydrous sodium sulfate to top of Ultra cartridge. Condition cartridge with 3 mL of acetonitrile. 0.100 mL (Load 0.750 mL of sample extract. Elute with 8 mL of acetontrile containing 0.5% formic acid. Collect eluent and concentrate at 40 °C to a final volume of )
clean-up tube Supelclean Ultra 2400, 3 mL (54281-U)
column SLB®-5ms, 30 m x 0.25 mm I.D., 0.250 μm (58471-U)
oven 105 °C (3 min), 10 °C/min to 130 °C, 4 °C/min to 200 °C, 8 °C/min. to 300 °C (10 min)
inj. temp. programmed: 100°C (.05 min), 720 °C/min to 280 °C (45 min)
detector MRM
MSD interface 300 °C
scan range MRM


Analysis Note Sample Prep: QuEChERS, Dispersive
Featured Industry Food and Beverages
General description Extraction tube: 4 mg/NaSO4, 1 g NaCl in 12 mL tube
Legal Information Supelclean is a trademark of Sigma-Aldrich Co. LLC, SLB is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for GC/MS


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