AOAC Method 2012.13: GC Analysis of Fatty Acids in Powdered Infant Formula on SP™-2560

AOAC Method 2012.13: GC Analysis of Fatty Acids in Powdered Infant formula on SP™-2560


sample/matrix weigh 168 mg powdered infant formula (approximately 50 mg of fat) into a 25 mL centrifuge tube; add 2 mL of water and mix to dissolve; (let sit for 15 minutes at room temperature; add 5 mL of internal standard (C11:0 FAME + C13:0 TAG, each at 2 mg/mL in methyl tert butyl ether))
extraction 5 mL of 5% (w/v) methanolic sodium methoxide solution; close tube and vortex for 10 seconds; after 180 seconds (time starts when sodium methoxide is added) add 2 mL of hexane; (after 210 seconds add 10 ml of neutralization solution (10% disodium hydrogen citrate/15% sodium chloride in water); gently shake using vortex mixer; centrifuge mixture at 1750 rpm for 5 minutes)
eluate post-treatment transfer 200 µL of supernatant into 10 mL flask and dilute to mark with hexane
column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056)
oven 60 °C (1 min), 15 °C/min to 165 °C (1 min), 2 °C/min to 225 °C (20 min)
carrier gas helium, 0.8 mL/min
injection 1µL, 10:1 split
liner 4 mm I.D., split/splitless type, wool packed single taper FocusLiner™ design
inj. temp. 250 °C
detector FID, 250 °C


Analysis Note The nutritional content of infant formula is extremely critical, and thus is one of the most regulated food products worldwide. Most commercial infant formulas are derived from cow’s milk, and are formulated to mimic human breast milk. They offer the advantage of being nutritionally balanced and easy for most babies to digest. All infant formulas, whether cow’s milk-based or from another source, are subject to specific regulatory nutritional content requirements. AOAC Method 2012.13 using a 100 m SP-2560 is able to provide identification of fatty acids, as well as determination of total fatty acid content and breakdown by class.
Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs)
Featured Industry Food and Beverages
Legal Information FocusLiner is a trademark of SGE Analytical Science Pty Ltd., SP is a trademark of Sigma-Aldrich Co. LLC
suitability application for GC


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