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GC Analysis of FAMEs in Atlantic Salmon on Omegawax®

GC Analysis of FAMEs in Atlantic Salmon on Omegawax®


column Omegawax, 30 m x 0.25 mm I.D., 0.25mum (24136)
oven 170 °C, 1 °C/min to 225C
carrier gas helium, 1.2 mL/min
sample 1. Fish Oil from capsule, prepared according to AOAC Method 991.39 2. Atlantic, farm-raised salmon, prepared according to AOCS Official Method Ce 1k-09
injection 1 µL, 100:1 split
liner 4 mm ID FocusLiner with taper
inj. temp. 250 °C
detector FID, 260 °C


Analysis Note Samples of farm-raised Atlantic salmon were prepared and analyzed for fatty acids. The salmon was extracted using an acid digestion /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (2). The resulting extracts were then analyzed by GC/FID on an Omegawax 250 °Capillary column as described in AOCS Official Method Ce 1i-07.
Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs)
Featured Industry Food and Beverages
Legal Information Omegawax is a registered trademark of Sigma-Aldrich Co. LLC
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