GC Analysis of Fresh and Rancid Potato Chips on SPB®-1 SULFUR after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

GC Analysis of Fresh and Rancid Potato Chips on SPB®-1 SULFUR after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber


sample/matrix 3 g crushed potato chips in 15 mL vial
desorption process 3 min, 250 °C
extraction headspace, 65 C, 20 min, with stirring
SPME fiber DVB/Carboxen/PDMS, 50/30 µm, StableFlex (57328-U)
column SPB-1 SULFUR, 30 m x 0.32 mm I.D., 4.0 µm (24158)
oven 45 °C (1.5 min) to 250 °C at 12 °C/min, hold 10 min
carrier gas helium, 40 cm/sec
sample 3g crushed potato chips in 15 mL vial
injection Splitless/split, closed 2 min
liner 0.75 mm liner
inj. temp. 250 °C
detector quadrupole mass spectrometer, m/z = 35-290 at 0.6 sec/scan


Analysis Note Potato chips can contain up to 50% oil and are subject to rancidity. Aldehydes, particularly hexanal and heptanal, are formed from fatty acids that are cleaved at the double bonds when exposed to ultraviolet light and air. A package of 3-year-old potato chips was analyzed using SPME and compared to a fresh package. The presence of aldehydes indicated that the older chips were rancid. However, due to the age of the chips, further oxidation of the aldehydes occurred that resulted in the formation of hexanoic and heptanoic acids. A Carboxen/PDMS/DVB fiber was inserted into the headspace of the potato chip samples at 65 °C for 20 minutes with agitation. The fiber was then analyzed using GC/MS on a SPB-1 SULFUR column. This non-polar column has a thick film and is ideal for smaller molecules.
Categories Analytical Chromatography, SPME Applications, GC Applications, Foods, Flavors & Fragrances, Volatiles
Featured Industry Food and Beverages
Legal Information SPB is a registered trademark of Sigma-Aldrich Co. LLC
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suitability application for SPME, application for GC


  • Acetic acid
  • Butanoic acid
  • Butyl hexanoate
  • Decanal
  • Dodecanal
  • Dodecanone
  • Heptanal
  • Heptanoic acid
  • Heptanone
  • Hexanal
  • Hexanoic acid
  • Methyl butyrate
  • Methyl heptanol
  • Methyl hexanal
  • Nonanal
  • Nonanone
  • Octanal
  • Octane
  • Octanoic acid
  • Pentanal
  • Pentyl hexanoate
  • Propyl acetate
  • Undecanone


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