GC Analysis of Glycerides in Butter on SLB®-35ms

GC Analysis of Glycerides in Butter on SLB®-35ms


column SLB-35ms, 10 m x 0.25 mm I.D., 0.10 µm; made by cutting down a 30 m x 0.25 mm I.D., 0.10 µm column (29802-U)
oven 75 °C (0.1 min), 15 °C/min to 380 °C (10 min)
carrier gas helium, 1.5 mL/min
sample butter, melted then diluted, 0.1% (v/v) in chloroform
injection 1µL, on-column
inj. temp. track oven: 75 °C (0.1 min), 15 °C/min to 380 °C (10 min)
detector FID, 380 °C


Analysis Note Triglycerides are the main constituent of vegetable oil and animal fat, and make up most of the fats digested by humans. Most natural fats contain a complex mixture of individual triglycerides. Therfore, efficient capillary GC columns are required for ample resolution and subsequent identification. Triglycerides are large compounds, and require a relatively high final oven temperature for GC analysis. For the analysis of triglycerides in butter, a short, thin film version of SLB-35ms (cut-down from a longer column) run to a final oven temperature of 380 ºC was found to provide good resolution and reasonable elution times.
Categories Analytical Chromatography, GC Applications, Glycerides
Featured Industry Food and Beverages
Legal Information SLB is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for GC


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