GC Analysis of Volatiles in Limoncello on SLB®-5ms after SPME using a 100 μm PDMS Fiber

GC Analysis of Volatiles in Limoncello on SLB®-5ms after SPME using a 100 μm PDMS Fiber


sample/matrix Limoncello diluted 1:100 in water, then 5 mL of this solution transferred to a 10 mL crimped vial spiked with 100 µL of a 100 ppm solution of nonane (internal standard)
desorption process 250 °C for 5 min
extraction headspace, with a pre-equilibration period of 10 min. at 40 °C, and then a fiber exposure of 30 min. at 40 °C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 ppm
SPME fiber 100 µm polydimethylsiloxane (57300-U)
column SLB-5ms, 30 m x 0.25 mm I.D., 0.25 µm (28471-U)
oven 40 °C, 3 °C/min to 250 °C (2 min)
carrier gas helium, 35.0 cm/sec
sample Limoncello liquor
injection splitless
liner 0.75 mm I.D. SPME (2633901)
inj. temp. 250 °C
detector FID, 260 °C (sampling rate 80 ms)


Analysis Note In this application, SPME was used for the analysis of volatiles in limoncello using a headspace method. SPME is ideal for the analysis of aroma volatiles in flavored beverages such as liquors. A 100 μm PDMS fiber was inserted into the headspace of the limoncello sample for 30 minutes at 40 °C followed by GC analysis using FID detection. The SLB-5ms GC columns provide consistently low-bleed, inert, efficient, and durable separations.
Categories Analytical Chromatography, Food & Beverage Analysis, SPME Applications, GC Applications, Foods, Flavors & Fragrances, Beverage Testing, Volatiles, Beer, Wine, and Spirits, Spirits
Featured Industry Food and Beverages
Legal Information SLB is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
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suitability application for SPME, application for GC


  • (E)-Caryophyllene
  • (E)-α-Bisabolene
  • (E)-β-Ocimene
  • (E)-γ-Bisabolene
  • (Z)-β-Ocimene
  • (Z)-β-Santelene
  • Bicycloelemene
  • Bicyclogermacrene
  • Camphene
  • Campherenol
  • cis-Limonene oxide
  • cis-Sabinene hydrate
  • Citronellal
  • Citronellyl acetate
  • Decanal
  • Geranial
  • Geraniol
  • Geranyl acetate
  • Geranyl propionate
  • Germacrene D
  • Isobornyl acetate
  • Limonene
  • Linalool
  • Methyl geranoate
  • Myrcene
  • Neral
  • Neryl acetate
  • Nonanal
  • Nonyl acetate
  • Octanol
  • Octyl acetate
  • p-Cymene
  • Perilla alcohol
  • Perilla aldehyde
  • Sabinene
  • Terpinen-4-ol
  • Terpinolene
  • trans-α-Bergamotene
  • Tricyclene
  • Undecanal
  • Valencene
  • α-Bisabolol
  • α-cis-Bergamotene
  • α-HuµLene
  • α-Phellandrene
  • α-Pinene
  • α-Terpinene
  • α-Terpineol
  • α-Thujene
  • β-bisabolene
  • β-Pinene
  • γ-Terpinene
  • δ-3-Carene
  • δ-Cadinene


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