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HPLC Analysis of a Synthetic Oligonucleotide on TSKgel(R) SuperQ-5PW

HPLC Analysis of a Synthetic Oligonucleotide on TSKgel® SuperQ-5PW


column TSKgel SuperQ-5PW, 7.5 cm x 7.5 mm I.D., 10 μm particles (818257)
mobile phase [A] 10 mM NaOH; [B] 10 mM NaOH, 1 M NaCl
gradient 0 to 50% B in 40 min; to 100% B in 1 min; held for 5 min
flow rate 1 mL/min
sample 16-mer morpholine oligonucleotide, AAG AAG AAG AGG GGA G
injection 0.5 O.D. (optical density)
detector UV, 254 nm


Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC


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