HPLC Analysis of Albuterol Tablets - Assay on Chromolith HighResolution RP-18 endcapped 50 x 4.6mm

HPLC Analysis of Albuterol Tablets - Assay on Chromolith HighResolution RP-18 endcapped 50 x 4.6mm

Conditions

sample preparation 120 μg/mL of Albuterol Sulfate RS prepared as follows. Transfer USP Albuterol Sulfate RS to a suitable volumetric flask, and add a volumne of Solution A corresponding to 60% of the flask volume. Sonicate for 5 minutes, and dilute with Methanol to volume.
column Chromolith® HighResolution RP-18 endcapped, 50 x 4.6 mm (1.52021.0001)
column temp. ambient
mobile phase [A] 10mL/L of glacial acetic acid in water; [B] 1.13g of sodium 1-Hexanesulfate in 1200mL water, add 12mL of glacial acetic acid; (36:64,:A:B)
flow rate 1.5 mL/min
pressure 1189 psi (82 bar)
sample Albuterol RS 30 μg/mL in diluent (30 μg/mL of Albuterol Sulfate RS in Diluent from Standard stock solution)
injection 8 μL
detector 276 nm cell: 11 μL

Description

Featured Industry Pharmaceutical (small molecule)
suitability application for HPLC

Materials

     
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