HPLC Analysis of Amlodipine Besylate in tablet on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Amlodipine Besylate in tablet on Chromolith® High Resolution RP-18e

Conditions

column Chromolith® High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. 25 °C
mobile phase [A] acetonitrile; [B] methanol; [C] 7.0 ml of triethylamine in 1000 ml Milli-Q® water. Adjust pH at 3.0 with ortho-phosphoric acid; (15:35:50, A:B:C)
flow rate 1.0 mL/min
sample amlodipine besylate (5 μg/mL) (Resolution solution: Weigh 5.0 mg of Amlodipine Besylate in 5.0 mL volumetric flask. Add 5.0 ml hydrogen peroxide. Heat it at 70°C for 45 mins in waterbath.)
injection 10 μL
detector 237 nm cell: 10 μL

Description

Analysis Note Preparation of Standards: Weigh 10 mg of amlodipine besylate in 20 mL volumetric flask. Add about 5.0 mL diluent and sonicate. Dilute up to the mark. Further dilute 1.0 mL to 100 mL with diluent.
Preparation of Tablets: Crush 20 tablets. Weigh powder equivalent to 5.0 mg amlodipine and transfer to a 50 mL volumetric flask. Add 30 mL diluent and sonicate for 20 mins. Dilute up to the mark.
Resolution solution: Weigh 5.0 mg of amlodipine besylate in a 5.0 mL volumetric flask. Add 5.0 mL hydrogen peroxide. Heat at 70°C for 45 mins in waterbath.
Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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