HPLC Analysis of Amlodipine Besylate Tablets on Purospher(R) STAR RP-18 endcapped

HPLC Analysis of Amlodipine Besylate Tablets on Purospher® STAR RP-18 endcapped


sample preparation Take 10 tablets into a 50-mL volumetric flask, add into 5mL mobile phase, sonicate for 30 minutes. Cool down solution to room temperature, add mobile phase to volume. (Shake well, filter, dilute with mobile phase to  a concentration of 50 μg/mL (50 ppm).)
column Purospher® STAR RP-18 endcapped 250 x 4.6 mm (1.51456.0001)
column temp. 30 °C
mobile phase [A] methanol; [B] acetonitrile; [C] 0.7% triethylamine aqueous solution ; (35:15:50, A:B:C)
flow rate 1 mL/min
sample Amlodipine Tablets
injection 10 μL
detector 237 nm 1 μL/ 10 mm


Featured Industry Pharmaceutical (small molecule)
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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