HPLC Analysis of Amlodipine & Olmesartan in tablet on Chromolith® High Resolution RP18e

HPLC Analysis of Amlodipine & Olmesartan in tablet on Chromolith® High Resolution RP18e

Conditions

column Chromolith® High Resolution RP18 endcapped, 100 x 4.6 mm (1.52022.0001)
mobile phase [A] 7.0 mL triethylamine in 1000 mL MilliQ water Adjust pH to 3.0 with dilute orthophosphoric.
Filter it with 0.2 μm filter; [B] acetonitrile; (65:35, A:B)
flow rate 1.0 mL/min
column temp. 40 °C
detector 237 nm cell: 10 μL
injection 10 μL
sample amlodipine (0.10 mg/mL), olmesartan (0.4 mg/mL)

Description

Categories Analytical/Chromatography, Antihypertensives, Applications, HPLC Applications
Analysis Note Standards: Weigh 10 mg of amlodipine and 40 mg of olmesartan standard in a 100 mL volumetric flask. Add about 50 mL diluent and sonicate until dissolved. Dilute up to the mark.
Tablets: Crush 20 tabl ets. Weigh equivalent of one tablet and transfer to a 100 mL volumetric flask. Add 50 mL diluent (buffer and acetonitrile, 65:35 v/v) and sonicate for 20 mins. Dilute up to the mark. Further dilute 5 mL of this solution to 50 mL. Filter through 0.2 μm filter.
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Materials

     
Related Links