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HPLC Analysis of Artificial Food Colorants in Orange Soft Drink on Chromolith® HighResolution RP-18 endcapped

HPLC Analysis of Artificial Food Colorants in Orange Soft Drink on Chromolith® HighResolution RP-18 endcapped

Conditions

column Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D., 2 µm particles (1.52022.0001)
mobile phase [A] 20mM ammonium acetate pH 6.8 in water; [B] methanol
gradient 5 to 50% B in 6 min; to 100% B in 4 min; held at 100% B for 1 min; to 5% B in 0.01 min
flow rate 1 mL/min
pressure 1526 psi (106 bar)
injection 20 µL
detector UV, 428 nm

Description

Categories Analytical Chromatography, Dyes
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes 5 mL of the beverage was transferred to a 10 mL volumetric flask, sonicated for 5 minutes, then pure water was added to the volume. The solution was filtered by 0.45 μm PTFE syringe filter into a 2mL autosampler vial prior to injection.
suitability application for HPLC

Materials

     
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