HPLC Analysis of Ascorbic acid on Chromolith(R) NH2 100 - 4.6mm

HPLC Analysis of Ascorbic acid on Chromolith® NH2 100 - 4.6mm


column Chromolith® NH2, 100 x 4.6 mm (1.52028.0001)
column temp. 25 °C
mobile phase [A] acetonitrile; [B] ammonium acetate 100mM pH=6.8; (90:10, A:B)
flow rate 4 mL/min
pressure 1566 psi (108 bar)
sample (Ascorbic acid 1mg/ml, Dehydroascorbic acid 0.5mg/ml)
injection 5 μL
detector 240 nm cell: 11 μL


Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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