HPLC Analysis of B-Trichothecenes on Ascentis® Express C18

HPLC Analysis of B-Trichothecenes on Ascentis® Express C18


column Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 µm particles (53814-U)
column temp. 35 °C
mobile phase [A] water:acetonitrile:methanol (92:4:4); [B] acetonitrile
gradient 0% B held for 5 minutes, to 8% B in 3 minutes, 8% B held for 2 minutes
flow rate 0.8 mL/min
pressure 4061 psi (280 bar)
sample 2 ppm each in water:methanol:acetonitrile (92:4:4)
injection 40 µL
detector UV, 220 nm


Analysis Note Mycotoxins are toxic secondary metabolites produced by fungi. Those chemicals exist in food as a result of fungal infection of crops. Mycotoxins have strong resistance to decomposition and digestion, so they remain in the food chain in meat and dairy products. They also resist to temperature, such as cooking and freezing. Their effects on human and animal health include death, cancer, weakened immune systems and as allergens or irritants.
Categories Analytical Chromatography, Mycotoxins
Featured Industry Agriculture
Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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