HPLC Analysis of Barbiturates on Ascentis® C18

HPLC Analysis of Barbiturates on Ascentis® C18


column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 35 °C
mobile phase [A] methanol; [B] water (50:50, A:B)
flow rate 1.0 mL/min
sample as indicated in 65:35 water:methanol
injection 10 µL
detector UV, 214 nm


Analysis Note Barbiturates are commonly abused and among the most widely tested compounds in clinical, forensic, or therapeutic drug monitoring applications. Shown here is the baseline separation of a set of barbiturates on an Ascentis C18 column. Highest grade HPLC solvents were used to supply low background interference and low particulate contamination for robust, trouble-free operation. Cerilliant and Sigma-Aldrich reference standards provided reliable identification and quantification.
Categories Analytical Chromatography, Forensics and Toxicology, Pharmaceutical Analysis, antiepileptic/antiseizure, Anticonvulsant/antiepiletic/antiseizure, drugs of abuse, Anxiolytics, Sedatives
Featured Industry Forensics and Toxicology
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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