HPLC Analysis of Barbiturates on Ascentis® C8

HPLC Analysis of Barbiturates on Ascentis® C8


column Ascentis C8, 15 cm x 4.6 mm I.D., 5 µm particles (581424-U)
column temp. 35 °C
mobile phase [A] methanol; [B] water (45:55, A:B)
flow rate 1.0 mL/min
sample 10 µg/mL each in 50:50 water:methanol
injection 5 µL
detector UV, 214 nm


Categories Analytical Chromatography, Forensics and Toxicology, Pharmaceutical Analysis, Anticonvulsant/antiepiletic/antiseizure, Drugs of Abuse, antiepileptic/antiseizure, drugs of abuse
Featured Industry Forensics and Toxicology
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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