HPLC Analysis of Betaine from Chinese Lycium on SeQuant(R) ZIC-HILIC using ELSD Detection

HPLC Analysis of Betaine from Chinese Lycium on SeQuant® ZIC-HILIC using ELSD Detection

Conditions

column SeQuant® ZIC-HILIC, PEEK, 250 x 4.6 mm, 5 μm, 200 Ã… (1.50458.0001)
mobile phase [A] methanol; [B] ammonium acetate; (75:25, A,B)
flow rate 1.0 mL/min
injection 20 μL raw sample diluted by 50% in water
detector ELSD (neb=40°C, evap=30°C, gas=1.6 SLM)

Description

Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for LC-MS

Materials

     
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