HPLC Analysis of Bimatoprost on Chromolith(R) Performance 100-3mm

HPLC Analysis of Bimatoprost on Chromolith® Performance 100-3mm


column Chromolith® Performance 100 x 3 mm (1.52001.0001)
column temp. ambient
mobile phase [A] acetonitrile; [B] water + 0,05% TFA v/v; [C] metanol; (30:60:10, A:B:C)
flow rate 850 μL/min
pressure 10827 psi (747 bar)
sample Bimatoprost (1mg/mL), Bimatoprost free acid (1mg/mL)
injection 2 μL
detector 210 nm cell: 1.4 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


Related Links