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HPLC Analysis of Bimatoprost on Chromolith(R) Performance 25-2mm

HPLC Analysis of Bimatoprost on Chromolith® Performance 25-2mm


column Chromolith® Performance, 25 x 2 mm (1.52014.0001)
column temp. ambient
mobile phase [A] acetonitrile; [B] water + 0,05% TFA v/v; [C] methanol; (25:70:5, A:B:C)
flow rate 190 μL/min
pressure 3898 psi (269 bar)
sample Bimatoprost (1mg/mL), Bimatoprost free acid (1mg/mL)
injection 1 μL
detector 210 nm cell: 1.4 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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