HPLC Analysis of Biogenic Amines on Ascentis® RP-Amide

HPLC Analysis of Biogenic Amines on Ascentis® RP-Amide


column Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 µm particles (565305-U)
column temp. 35 °C
mobile phase [A] 0.1% ammonium acetate in water (pH unadjusted); [B] acetonitrile
gradient 25 to 75% B in 10 min; held at 75% B for 5 min
flow rate 0.5 mL/min
injection 10 µL
detector UV, 340 nm


Analysis Note Following pre-column derivatization of primary amino groups using o-phthaldialdehyde/thiol reagent, the Ascentis RP-Amide was used for the analysis of biogenic amines commonly found in wines. These include histamine, spermine, spermidine, tyramine, putrescine and cadaverine. Structures along with an optimized chromatogram are presented.
Categories Analytical Chromatography, Clinical, amines, Proteins, proteins
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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