HPLC Analysis of Bisphenol A in Milk Powder on Chromolith® High Resolution RP-18e

HPLC Analysis of Bisphenol A in Milk Powder on Chromolith® High Resolution RP-18e

Conditions


Sample Prep Method

 

filter description Millex® Samplicity filters, 0.20 µm, hydrophilic, PTFE; Bold blue Samplicity system bundle with 0.2 µm PTFE Millex® Samplicity filters


Primary Analytical Method

 

instrument Hitachi Chromaster-5440 Fluorescence Detector
column Chromolith High Resolution RP-18 endcapped  10 cm x 4.6 mm I.D.
mobile phase   [A] water; [B] acetonitrile; (75:25, A:B)
flow rate 2 mL/min (0 - 4 min), 4 mL/min (4.1 - 7.5 min), 2 mL/min (7.6  - 8  min)
pressure 90-185 bar (1305-2682.5 psi)
column temp. 35 °C
detector fluorescence, λex=230; λem=316 nm, 13 µL cell
injection 10 µL

Description

Analysis Note To facilitate the monitoring of BPA we have developed a rapid and inexpensive method for the analysis of baby milk formulations, and the data presented here illustrates the excellent long term performance and method robustness obtained with Chromolith® columns. More than 2700 samples were analyzed on a single column.
Featured Industry Food & Beverage
Legal Information Chromolith is a trademark of Merck KGaA, Darmstadt, Germany
Millex is a trademark of Merck KGaA, Darmstadt, Germany
Samplicity is a trademark of Merck KGaA, Darmstadt, Germany
Application No. ASC290118

Materials

     
Related Links