HPLC Analysis of Breviscapine on Purospher(R) STAR RP-18 endcapped

HPLC Analysis of Breviscapine on Purospher® STAR RP-18 endcapped


column Purospher® STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)
column temp. 30 °C
mobile phase [A] 1% glacial acetic acid solution; [B] acetonitrile; (83:17, A:B)
flow rate 1 mL/min
pressure 1595 psi 110 Bar
sample Weigh Scutellarin, dissolve with methanol to obtain 30 μg/mL (30 ppm) of standard solution.
injection 10 μL
detector 335 nm cell: 1 μL/10 mm


Featured Industry Pharmaceutical (small molecule)
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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