HPLC Analysis of Breviscapine tablets on Purospher(R)STAR RP-18 endcapped)

HPLC Analysis of Breviscapine tablets on Purospher®STAR RP-18 endcapped)


sample preparation Take 10 tablets, weigh precisely, grind to powder. Weight appropriate amount (roughly contained 30mg Breviscapine) to a 50 mL volumetric flask, add methanol into, sonicate for 30 minutes (250W, 40kHZ). Cool down, add methanol to volume. Shake well, filtrate. Take 1 mL filtrate to a 25 mL volumetric flask, add methanol to volume.
column Purospher® STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)
column temp. 30 °C
mobile phase [A] 1% glacial acetic acid solution; [B] acetonitrile; (83:17, A:B)
flow rate 1 mL/min
pressure 1595 psi 110 Bar (110 Bar)
sample Weigh Scutellarin, dissolve with methanol to obtain 30 μg/mL (30 ppm) of standard solution.
injection 10 μL
detector 335 nm cell: 1 μL/ 10 mm


Featured Industry Pharmaceutical (small molecule)
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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