HPLC Analysis of Caffeine and Theophylline on SeQuant ZIC-HILIC

HPLC Analysis of Caffeine and Theophylline on SeQuant ZIC-HILIC

Conditions

column Sequant ZIC-HILIC, PEEK, 15 cm x 4.6 mm, 5 μm, 200 Å
mobile phase [A] acetonitrile; [B] 100 mM ammonium acetate, pH 4.5; (90:10, A,B) (total ionic strength 10 mM)
flow rate 0.1 mL/min
pressure 2.8 MPa (403 psi)
detector UV, 254 nm
injection 5 µL

Decription

Featured Industry Food & Beverage
Legal Information SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
Analysis Note Analytical method for rapid determination using HILIC mode chromatography.
Application No. ASC120118E

Materials

     
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