HPLC Analysis of Cannabinoids using Ascentis® Express RP-Amide

HPLC Analysis of Cannabinoids using Ascentis® Express RP-Amide


column Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (50774-U)
column temp. 35 °C
mobile phase 5 mM ammonium acetate (pH 4.5 with acetic acid) in 20:80, acetonitrile:water
flow rate 1.0 mL/min
pressure 1450 psi (100 bar)
sample each compound 50 µg/mL in water:methanol (50:50)
injection 5 µL
detector UV, 214 nm


Analysis Note The rapid growth of the cannabis testing market has prompted the development of equally rapid methods to analyze the active ingredients. Shown here is the separation of cannabis compounds on an Ascentis Express RP-Amide column. The selectivity, and therefore resolution, is unique to the amide bonded phase chemistry. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Forensics and Toxicology, drugs of abuse, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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