HPLC Analysis of Carbidopa on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Carbidopa on Chromolith® High Resolution RP-18e


column Chromolith® High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. ambient
mobile phase [A] methanol; [B] water + 0,1%TFA;
gradient 100% B held for 0.01 min; to 80% B in 0.9 min; held at 80% A for 9 min (Original: Initial composition: 0-0.1min 100%B, 1-10min 100%B to 80%B)
flow rate 2.5 mL/min
sample 2,4,5-Trihydroxyphenylalanine 4μg/ml, Levodopa 402μg/ml, Methyldopa 384μg/ml, Daopamine 217μg/ml, Carbidopa 232μg/ml, 3,4-Dihydroxyphenyl acetic acid 4μg/ml, 3-o-Methylcarbidopa 4μg/ml
injection 1 μL
detector 282 nm


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


Related Links