HPLC Analysis of Cefixime and Ofloxacin in Tablets on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Cefixime and Ofloxacin in Tablets on Chromolith® High Resolution RP-18e

Conditions

sample preparation Crush 20 tablets. Weigh equivalent to one tablet quantity & transfer to 100 mL volumetric flask. Add 50 ml diluent & sonicate for 20 mins. Dilute up to the mark the same. Further dilute with 2.5 mL of this solution to 100 mL. Filter through 0.2 μm filter
column Chromolith® High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. 25 °C
mobile phase [A] 3.4 g of potassium dihydrogen phosphate in 1000 ml MilliQ water. Mix & sonicate it. Filter it with 0.2 μm filter; [B] acetonitrile; 87:13, A:B)
flow rate 1.0 mL/min
pressure 764 psi (53 bar)
sample Weigh 5.0 mg of Cefixime & 5.0 mg of Ofloxacin refernce standard in 100 mL volumetric flask. Add about 50 mL diluent & sonicate till it dissolve. Dilute it upto the mark with the same. (Diluent: Dissolve 7.1 g of di-sodium hydrogen phosphate in 500 ml MilliQ water. Adjust pH at 7.0 with ortho-phosphoric acid. Mix & sonicate it. Filter it with 0.2 μm filter.)
injection 5 μL
detector 290 nm cell: 10 μL

Description

Analysis Note Sample: 50 ppm (0.050 mg/mL) of each cefixime and ofloxacin in diluent.
Diluent: 7.1 g of disodium hydrogen phosphate in 500 mL Milli-Q water. pH 7.0 with orthophosphoric acid.
Method developed in house for tablet analysis.
Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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