HPLC Analysis of Cetirizine Hydrochloride Tablets on Purospher(R) STAR RP-18 endcapped

HPLC Analysis of Cetirizine Hydrochloride Tablets on Purospher® STAR RP-18 endcapped

Conditions

sample preparation Weigh appropriate amount of Cetirizine, dissolve with H2O to obtain 100 μg/mL (100 ppm) solution. Take 10 Cetirizine Hydrochloride tablets, weigh precisely, grind to powder. Weight appropriate amount to a 100-mL volumetric flask, add appropriate amount of H2O. Sonicate, add H2O to volume. Filtrate.
column Purospher STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)
column temp. 30 °C
mobile phase [A] 100mM NaH2PO4 aqueous solution, adjusted to pH 3.7 with H3PO4; [B] acetonitrile; (60:40, A:B)
flow rate 1.0 mL/min
pressure 1465 psi (101 Bar)
sample Weigh appropriate amount of Cetirizine, add diluent to obtain 100 μg/mL (100 ppm) concentration.
injection 20 μL
detector 232 nm cell: 1 μL/10 mm

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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