HPLC Analysis of Contents of Energy Drinks on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Contents of Energy Drinks on Chromolith® High Resolution RP-18e

Conditions

sample preparation 1ml Sample on Extrelut N1, wait 10min., eluate with 6ml Ethylacetate, (blow up Ethylacetate with nitrogen, dillute sample in 1ml water )
column Chromolith® Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. 40 °C
mobile phase [A] methanol; [B] 20mM Phosphat buffer pH=5; (10:90, A:B)
gradient 0-3min 1mL/min, 3,1-12min 2,5mL/min, 12,1-15min 1mL/min
flow rate 0-3min 1mL/min, 3,1-12min 2,5mL/min, 12,1-15min 1mL/min
sample Energy drink (Red Bull)
injection 8 μL
detector 227 nm (cell: 11 μL)

Description

Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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