HPLC Analysis of Diclofenac and Metabolits on Chromolith(R) High Resolution RP-18e

HPLC Analysis of Diclofenac and Metabolits on Chromolith® High Resolution RP-18e


sample preparation Diclofenac was solved in 10mM Sodiumphasphatbuffer pH=3,5 and stired over night by 40°C. Then ACN was added to get a Mixture of ACN/buffer 50/50 v/v
column Chromolith High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)
column temp. ambient
mobile phase [A] acetonitrile; [B] sodium-phosphatbuffer 10mM pH=3,5; (50:50, A:B)
flow rate 1.0 mL/min
sample Diclofenac 0,1mg/ml
injection 5 μL
detector 280 nm cell: 11 μL


Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC


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