HPLC Analysis of Dioxibrassinin Enantiomers on Astec® P-CAP™

HPLC Analysis of Dioxibrassinin Enantiomers on Astec® P-CAP™


column Astec (R,R) P-CAP-DP, 25 cm x 4.6 mm I.D., 5 µm particles (35024AST)
column temp. 25 °C
mobile phase [A] acetonitrile; [B] methanol; (97:3, A:B)
flow rate 1 mL/min
sample 1 mg/mL in methanol
injection 1 µL
detector UV, 255 nm


Analysis Note A crucifer phytoalexin, along with brassinin, cyclobrassinin, brassicanals A and C. Phytoalexins are essential secondary metabolites produced de novo by plants in response to diverse forms of stress, including microbial attack.
Categories Analytical Chromatography, Chiral Applications, Vitamins, Nutraceuticals & Natural Products, Environmental, Life Science & Biopharma, Pesticides & Herbicides, Other Biochemical Applications, Other Environmental Applications, All Biochemical Applications, All Environmental Applications
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information P-CAP is a trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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