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HPLC Analysis of Diuretic Drugs on Ascentis® C18

HPLC Analysis of Diuretic Drugs on Ascentis® C18


column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 35 °C
mobile phase [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] acetonitrile; (A:B, 40:60)
flow rate 1 mL/min
sample 10 µg/mL each in mobile phase
injection 10 µL
detector UV, 234 nm


Categories Analytical Chromatography, Diuretics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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