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HPLC Analysis of Drugs of Abuse on Ascentis® Phenyl

HPLC Analysis of Drugs of Abuse on Ascentis® Phenyl


column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 °C
mobile phase [A] water with 0.1% ammonium acetate, pH 6.6, unadjusted; [B] acetonitrile with 0.1% ammonium acetate
gradient 25 to 40% B in 7 min; to 25% B in 1 min
flow rate 1.0 mL/min
sample as indicated in mobile phase A
injection 10 µL
detector UV, 280 nm


Categories Analytical Chromatography, Analgesics
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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