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HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange

HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange

Conditions

column Discovery BIO PolyMA-SCX, 5cm x 4.6mm, 5mum particles (59601-U)
column temp. 25 °C
mobile phase [A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0
gradient 0 to 100% B in 30 min. (linear)
flow rate 0.5 mL/min
injection 10 µL
detector UV, 280 nm

Description

Analysis Note Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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